How does Chromperfect Unite work? Chromperfect Unite shares the same core software as the fully fledged versions of the product. The user can open data from any other software system using the seamless and automatic file import system to allow data files to be opened directly within Chromperfect Unite. All references to other trademarks belonging to third parties or referring to branded software or instruments, shall be understood to refer to those registered trademarks owned by others, and not to any trademark belonging to Justice innnovations Inc.
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Hi antonk, Can you please contact me via email john. I have some information on the standalone software that I can share with you, including a link that your FSE can use to download the software. Previous topic. Next topic. Who is online.
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Similar difficulties can be faced when a laboratory reproduces methods from pharmacopeia monographs, typically on instruments different from those used for the monograph publication. This means you change the column format often 4.
In such case, the gradient table, injection volume and flow rate need to be changed. To accomplish this there are great online tools avaialble will do the match for you. In Chromeleon CDS you can calculate these change directly from your existing method and apply this new method to you instrument.
However, if you would like to use the identical column 4. Becasue the dispersion of a modern UHPLC instrument is way less than stnadrd HPLC instrument you need to take care of any solvent mismatches that might occure when for instance injecting high organic samples stronger elution strength than eluent.
In addition, due to the narrow tubing care should be taken to only work with really clean and completely dissolved ssamples oion order to avoid clogging of the tubing. How to decrease the sample concentration and volume for analysis if we have small amount of prepared samples?
There are multiple ways how to reduce the amount of sample needed for the analysis with a liquid chromatograph. Reduce simply the injection volume while simultaeously using high recovery vials.
Using the most sensitive UV detectors e. Light pipe technology or changing to an alternative detection technology Fluorescence, Charged Aerosol, mass detection can help to increase the sensitivty. Concrete guidelines would depend analyte physical properties.
Lower injection volumes are generally needed when going from standard bore 4. Within the portfolio of Thermo Scientific LC instrument we have instruments for all of these applications use Chromeleon or online calculator The common LC Method Transfer Calculator only able to convert between column type, method condition such as flow and gradient table of mobile phase. To develop the most sensitive FLD detection method our fluorescence detectors enable a 3D flied acquisition to determine optimal excitation and emmission wavelength.
Detailed guidelines can be found in this method development handbook. What is the challenge to get good accuracy during the method transfer involving instruments from different vendors or with different configurations? In order to match the previous analysis on a new instrument e. A Vanquish Core instrument, we implemented a couple of unique features to make that transfer as easy as possible.
Variable column thermostatting options 3. Custom injection programs to deal with high organic strength samples Can HPLC can be applied for the invention of new drugs? HPLC is a tool that is extremly widely used in pharmaceutical research. This technique is needed to qualitatively and quantitatively characterize samples. This includes drug candidate analysis with often an LC-MS instrument as well as to control the quality during pharmaceutical production challenges while analytical method developed on UPLC and transferring on normal HPLC system.
Transferring a method that was originally developed on an UHPLC instrument and now needs to be transferred to a standard HPLC instrument can be challenging but depends on the used column format. However, if the method is based for instance on a 2. The most severe challenges will be the overall system pressure depending on column length as well as the system dispersion.
For the post column dispersion especially the flow cell volume needs attention. It needs to be checked if the HPLC instrument is able to deal with this. This instrument as pressure limit of bars, post column dispersion characteristics can be easily changes by using fingertight Viper fitting and low volume flow cells and the autosampler is able to inject typical UHPLC sample volumes.
Method and condition replication to achieve robustness in another laboratory for method validation of a newly developed analysis method. The Chromeleon ICH Method Validation pack provides everything you need to perform reprodcibility and rebostness testing with predifined and automoated reporting during your method development process. In addition, dedicated software tools, for instance AutoRobust from Chromsword, are tested to work with Thermo Scientific LC instruments and accelerate the method development process How does sample pre mixing with mobile phase help improve the peak shape?
Sample premixing aims to reduce the elution power of the sample which is a common cause for poor peak shapes, besides any column effects. With a lower elution power the sample can be focused on the column head. With this dilution approach the analyte concentration will be decreased and thus need a higher injection volume.
A best first appropach would be to keep the mass on column of the analyte constant. Care most be taken that the analytes are still soluble in the weaker mobile phase and do not preticipate What's the most remarkable thing about this technology? Vanquish Core has several features to facilitate the transfer of methods, so bringing an existing method to a new instrument such as the Vanquish Core.
This is: - a freely tunable gradient delay volume to match the gradient delay volume of other common HPLC instruments - multiple column thermostatting options to mimic other vendor thermostatting concepts - Custom Inject Programs for in needle sample dilution prior to injection In the case of analysis of additives in emulsion and aqueous samples, there are difficulties such as insolubility and precipitation during pretreatment.
Please advise any helpful tips or measurement considerations. For liquid chromatography it is mandatory to have the analyte dissolved. Thermodynamic and kinetic considerations need to be taken. Common advices such as ultrosonic bath, heating etc need to be evaluated in a case by case study How effective is method transfer for herbal products? The challenge for herbal products is the complexity of the sample and chromatogram. Vanquish Core is the best instrument for such transfer as it comes with a unique feature set to make the method transfer as easy as possible How do we identify the method used in sample running?
Chromeleon CDS provides a sequence table with all samples used and the respective instrument methods. Chromeleon audit trail features will log all this data so that any time the instrument details for a particular sample can be reviewed. Can Thermo help to study the comparsion report and validate? For detailed answers we would need to get more details. Contact our HPLC experts now. How to overcome the issue of sample denaturation due to less stable in unfavorable environment in HPLC?
The common causes that accelerate sample degradation are temperature, light induced oxidation or oxidation caused by air oxygen.
To remove the temperature effect all our autosampler can be ordered with a very effective cooling option. Minimizing the light effect can be done by turning of the light in the autosampler or using brown glass vials. For oxidation caused by oxygen is probably the hardest to fix. Filling the vials under an nitrogen atmosphere might help here. Quality of vials and closures are also something to consider.
Thermo Scientific offers a broad range of excellent vials, well plates and closures. How to minimize the deviations in the chromatograms? Are there usefull ways to make the chromatogram always consistent? There are three considerations I would list: 1. Differences in gradient delay volume. Here Vanquish Core is the only instrument available that can physically change the gradient delay volume seemlessly on a microliter scale 2. Differences in pre or post column dispersion.
For pre-column dispersion Vanquish LC systems offer Customer Injection Programs to to for instance dilute high orgnic samples. For post column dispersion, the biggest influence comes from the flow cell.
Here we provide different flow cell volumes 3. This is the mode of thermostatting but also if the eluent is pre-heated or not. Vanquish Core offer multiple heating modes and pre-heating option to mimic all of these What are the top three considerations when transferring a method between HPLCs of different manufactures?
In general there are no regulatory guidelines on which parameter need to be measured. In any case, it is important to work out beforehand a clear method trasnfer plan with pre-defined acceptance criteria such as area precision, LOD, linearity, robustness, specificty etc. Also statistical analysis are important and different approaches exists.
The Chromeleon ICH Method Validation pack provides everything you need to perform reprodcibility and rebustness testing with predifined and automoated reporting during your method development process. What are some of the basic instrument care that have to be taken into consideration when we do method transfer?
When doing a method transfer with Vanquish Core no special care has to be taken for instrument. This part will help to have a broader range of gradient delay volume for mimicing other instruments. Thus we recommend this optional part for the purpose of method transfer. What are some ways for method and condition replication to achieve robustness in another laboratory for method validation of a newly developed analysis method? We recommend thorough robustness testing during the method velopment process.
Typical parameters to check are influence of varifying pH value, different column lots etc What are regulatory guidelines over method transfers between various vendor instruments? For the approach with Vanquish Core the gradient delay volume can be altered with any need for re-qualification of the instrument as now part are exchanged.
The settings to change the gradient delay volume are tracked in the instrument audit trail. Chromatography Data systems has can integrate peaks in multiple ways. This way difference comming for instance from a valley to valley or drop perpendiculum integration can be directly compared.
Panelists: Dr. Cook Thermo Fisher Scientific. Well developed Software. Application Area: Pharmaceutical company. Very powerful report tools excel like allow the creation of the fanciest report you would want. I was sold to Empower before but now would never go back. After sale support is amazing. Software is user friendly and intuitive. Worst chromatography software I've ever used. Rating: 1. The inability to generate and save a method independently of a sequence is annoying and getting each sequence to run once set up is proving hugely frustrating.
How did simple and effective chromatography software evolve into something this horrendous? Best chromatography software to use. Application Area: Data analysis of analytes. Recommend this to all labs.
Application Area: Ion Chromatography. I was especially impressed with the help and training that I received during and shortly after the installation.
I've seen better. Application Area: analyze small molecules. However, the software is not too user- friendly. Great results.
Application Area: Protein extraction; Protein Chromatography. Very reliable instrument. Application Area: Anion analysis in waste water method I find it a little confusing, but I chose not to take much training for it. With only 2 hours of webinar training I am able to do everything I need to do. The software will do much more that what I need.
Reproducible and great results. Application Area: Analyze drugs. The data system software is easy to use because it is intuitive. Easy to use versatile chromatography software package.
Application Area: GC analysis of catalytically derived biofuels, chemicals and intermediates. The versatility allows us to limit the number of different software packages that are needed for all of our analysis improving analyst efficiency.
Chromeleon has been a stable software package for us and any issues that we have had have been promptly addressed but the service team. Chromeleon just got better. Application Area: Pharmacuticals. Willmar Schwabe Ease of use 4 out of 5 After sales service 5 out of 5 Value for money 5 out of 5. Application Area: Analyze Phyto-Pharmaceutics. Easy to use;. Application Area: Analyze Anions in Coal.
It is easy to handle the data especially if I must reintegrate the chromatogram. Easy to use. Application Area: Analysis of Environmental samples. Application Area: Analyze metabolites in tissue samples and monitor enzymatic reactions.
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